IDH-1 - Revision history

Jensflorian: /* Negative */ update

2018-03-14T14:13:43Z

Negative: update

← Older revision		Revision as of 14:13, 14 March 2018
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	*[[Pilocytic astrocytoma]]		*[[Pilocytic astrocytoma]]
	*Neuronal tumors		*Neuronal tumors
	*Sarcoma, carcinoma <ref>{{Cite journal \| last1 = Sahm \| first1 = F. \| last2 = Capper \| first2 = D. \| last3 = Meyer \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Herpel \| first5 = E. \| last6 = Andrulis \| first6 = M. \| last7 = Mechtersheimer \| first7 = G. \| last8 = Petersen \| first8 = I. \| last9 = Paulus \| first9 = W. \| title = Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation. \| journal = Histopathology \| volume = 58 \| issue = 7 \| pages = 1167-72 \| month = Jun \| year = 2011 \| doi = 10.1111/j.1365-2559.2011.03823.x \| PMID = 21707716 }}</ref>		*Sarcoma, carcinoma <ref>{{Cite journal \| last1 = Sahm \| first1 = F. \| last2 = Capper \| first2 = D. \| last3 = Meyer \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Herpel \| first5 = E. \| last6 = Andrulis \| first6 = M. \| last7 = Mechtersheimer \| first7 = G. \| last8 = Petersen \| first8 = I. \| last9 = Paulus \| first9 = W. \| title = Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation. \| journal = Histopathology \| volume = 58 \| issue = 7 \| pages = 1167-72 \| month = Jun \| year = 2011 \| doi = 10.1111/j.1365-2559.2011.03823.x \| PMID = 21707716 }}</ref> - note that some Cholangiocarcinoma contain IDH1 R132C or R172K mutations.<ref>{{Cite journal \| last1 = Farshidfar \| first1 = F. \| last2 = Zheng \| first2 = S. \| last3 = Gingras \| first3 = MC. \| last4 = Newton \| first4 = Y. \| last5 = Shih \| first5 = J. \| last6 = Robertson \| first6 = AG. \| last7 = Hinoue \| first7 = T. \| last8 = Hoadley \| first8 = KA. \| last9 = Gibb \| first9 = EA. \| title = Integrative Genomic Analysis of Cholangiocarcinoma Identifies Distinct IDH-Mutant Molecular Profiles. \| journal = Cell Rep \| volume = 18 \| issue = 11 \| pages = 2780-2794 \| month = Mar \| year = 2017 \| doi = 10.1016/j.celrep.2017.02.033 \| PMID = 28297679 }} not detected by IHC.</ref>

	==Functional meaning==		==Functional meaning==

Jensflorian: /* Tumours */ chondrosarcoma added

2018-03-14T14:08:34Z

Tumours: chondrosarcoma added

← Older revision		Revision as of 14:08, 14 March 2018
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	==Tumours==		==Tumours==
	*[[Oligodendroglioma]]<ref>{{Cite journal \| last1 = Capper \| first1 = D. \| last2 = Reuss \| first2 = D. \| last3 = Schittenhelm \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Bremer \| first5 = J. \| last6 = Sahm \| first6 = F. \| last7 = Harter \| first7 = PN. \| last8 = Jeibmann \| first8 = A. \| last9 = von Deimling \| first9 = A. \| title = Mutation-specific IDH1 antibody differentiates oligodendrogliomas and oligoastrocytomas from other brain tumors with oligodendroglioma-like morphology. \| journal = Acta Neuropathol \| volume = 121 \| issue = 2 \| pages = 241-52 \| month = Feb \| year = 2011 \| doi = 10.1007/s00401-010-0770-2 \| PMID = 21069360 }}</ref>		*[[Oligodendroglioma]] <ref>{{Cite journal \| last1 = Capper \| first1 = D. \| last2 = Reuss \| first2 = D. \| last3 = Schittenhelm \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Bremer \| first5 = J. \| last6 = Sahm \| first6 = F. \| last7 = Harter \| first7 = PN. \| last8 = Jeibmann \| first8 = A. \| last9 = von Deimling \| first9 = A. \| title = Mutation-specific IDH1 antibody differentiates oligodendrogliomas and oligoastrocytomas from other brain tumors with oligodendroglioma-like morphology. \| journal = Acta Neuropathol \| volume = 121 \| issue = 2 \| pages = 241-52 \| month = Feb \| year = 2011 \| doi = 10.1007/s00401-010-0770-2 \| PMID = 21069360 }}</ref>
	*[[Astrocytoma]]		*[[Astrocytoma]]
	*Secondary [[glioblastoma]]		*Secondary [[glioblastoma]]
	*[[Myelodysplasia]]<ref>{{Cite journal \| last1 = Andrulis \| first1 = M. \| last2 = Capper \| first2 = D. \| last3 = Luft \| first3 = T. \| last4 = Hartmann \| first4 = C. \| last5 = Zentgraf \| first5 = H. \| last6 = von Deimling \| first6 = A. \| title = Detection of isocitrate dehydrogenase 1 mutation R132H in myelodysplastic syndrome by mutation-specific antibody and direct sequencing. \| journal = Leuk Res \| volume = 34 \| issue = 8 \| pages = 1091-3 \| month = Aug \| year = 2010 \| doi = 10.1016/j.leukres.2010.02.014 \| PMID = 20227112 }}</ref>		*[[Myelodysplasia]] <ref>{{Cite journal \| last1 = Andrulis \| first1 = M. \| last2 = Capper \| first2 = D. \| last3 = Luft \| first3 = T. \| last4 = Hartmann \| first4 = C. \| last5 = Zentgraf \| first5 = H. \| last6 = von Deimling \| first6 = A. \| title = Detection of isocitrate dehydrogenase 1 mutation R132H in myelodysplastic syndrome by mutation-specific antibody and direct sequencing. \| journal = Leuk Res \| volume = 34 \| issue = 8 \| pages = 1091-3 \| month = Aug \| year = 2010 \| doi = 10.1016/j.leukres.2010.02.014 \| PMID = 20227112 }}</ref>
	*[[Acute myeloid leukemia]]		*[[Acute myeloid leukemia]]
			*[[Chondrosarcoma]] <ref>{{Cite journal \| last1 = Amary \| first1 = MF. \| last2 = Bacsi \| first2 = K. \| last3 = Maggiani \| first3 = F. \| last4 = Damato \| first4 = S. \| last5 = Halai \| first5 = D. \| last6 = Berisha \| first6 = F. \| last7 = Pollock \| first7 = R. \| last8 = O'Donnell \| first8 = P. \| last9 = Grigoriadis \| first9 = A. \| title = IDH1 and IDH2 mutations are frequent events in central chondrosarcoma and central and periosteal chondromas but not in other mesenchymal tumours. \| journal = J Pathol \| volume = 224 \| issue = 3 \| pages = 334-43 \| month = Jul \| year = 2011 \| doi = 10.1002/path.2913 \| PMID = 21598255 }}</ref>

	===Negative===		===Negative===

Jensflorian: update

2018-03-14T14:05:50Z

update

← Older revision		Revision as of 14:05, 14 March 2018
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	*Neuronal tumors		*Neuronal tumors
	*Sarcoma, carcinoma <ref>{{Cite journal \| last1 = Sahm \| first1 = F. \| last2 = Capper \| first2 = D. \| last3 = Meyer \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Herpel \| first5 = E. \| last6 = Andrulis \| first6 = M. \| last7 = Mechtersheimer \| first7 = G. \| last8 = Petersen \| first8 = I. \| last9 = Paulus \| first9 = W. \| title = Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation. \| journal = Histopathology \| volume = 58 \| issue = 7 \| pages = 1167-72 \| month = Jun \| year = 2011 \| doi = 10.1111/j.1365-2559.2011.03823.x \| PMID = 21707716 }}</ref>		*Sarcoma, carcinoma <ref>{{Cite journal \| last1 = Sahm \| first1 = F. \| last2 = Capper \| first2 = D. \| last3 = Meyer \| first3 = J. \| last4 = Hartmann \| first4 = C. \| last5 = Herpel \| first5 = E. \| last6 = Andrulis \| first6 = M. \| last7 = Mechtersheimer \| first7 = G. \| last8 = Petersen \| first8 = I. \| last9 = Paulus \| first9 = W. \| title = Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation. \| journal = Histopathology \| volume = 58 \| issue = 7 \| pages = 1167-72 \| month = Jun \| year = 2011 \| doi = 10.1111/j.1365-2559.2011.03823.x \| PMID = 21707716 }}</ref>

			==Functional meaning==
			The R132H amino acic exchange alters the enzymatic activity of IDH resulting in production D-2-hydroxyglutarate (D-2HG).<ref>{{Cite journal \| last1 = Longuespée \| first1 = R. \| last2 = Wefers \| first2 = AK. \| last3 = De Vita \| first3 = E. \| last4 = Miller \| first4 = AK. \| last5 = Reuss \| first5 = DE. \| last6 = Wick \| first6 = W. \| last7 = Herold-Mende \| first7 = C. \| last8 = Kriegsmann \| first8 = M. \| last9 = Schirmacher \| first9 = P. \| title = Rapid detection of 2-hydroxyglutarate in frozen sections of IDH mutant tumors by MALDI-TOF mass spectrometry. \| journal = Acta Neuropathol Commun \| volume = 6 \| issue = 1 \| pages = 21 \| month = 03 \| year = 2018 \| doi = 10.1186/s40478-018-0523-3 \| PMID = 29499756 }}</ref> Therefore the mutated enzyme is considered an oncometabolite.
			[[File:Wild-type_and_mutant_IDH1_structures.png\|400px]]

Jensflorian at 15:05, 14 October 2015

2015-10-14T15:05:21Z

← Older revision		Revision as of 15:05, 14 October 2015
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	[[Category:Immunohistochemistry]]		[[Category:Immunohistochemistry]]
			[[Category:Neuropathology]]

Jensflorian: /* Positive staining */ pic update

2015-10-06T08:10:53Z

Positive staining: pic update

← Older revision		Revision as of 08:10, 6 October 2015
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	==Positive staining==		==Positive staining==
	*Cytoplasmic<ref>{{Cite journal \| last1 = Capper \| first1 = D. \| last2 = Weissert \| first2 = S. \| last3 = Balss \| first3 = J. \| last4 = Habel \| first4 = A. \| last5 = Meyer \| first5 = J. \| last6 = Jäger \| first6 = D. \| last7 = Ackermann \| first7 = U. \| last8 = Tessmer \| first8 = C. \| last9 = Korshunov \| first9 = A. \| title = Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. \| journal = Brain Pathol \| volume = 20 \| issue = 1 \| pages = 245-54 \| month = Jan \| year = 2010 \| doi = 10.1111/j.1750-3639.2009.00352.x \| PMID = 19903171 }}</ref>		*Cytoplasmic<ref>{{Cite journal \| last1 = Capper \| first1 = D. \| last2 = Weissert \| first2 = S. \| last3 = Balss \| first3 = J. \| last4 = Habel \| first4 = A. \| last5 = Meyer \| first5 = J. \| last6 = Jäger \| first6 = D. \| last7 = Ackermann \| first7 = U. \| last8 = Tessmer \| first8 = C. \| last9 = Korshunov \| first9 = A. \| title = Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. \| journal = Brain Pathol \| volume = 20 \| issue = 1 \| pages = 245-54 \| month = Jan \| year = 2010 \| doi = 10.1111/j.1750-3639.2009.00352.x \| PMID = 19903171 }}</ref>
			** Does not stain endothelial cells and inflammatory cells.

	<gallery>		<gallery>
	File:IDH1_GBM_20x.jpg \| IDH-1 (R132H) in a secondary glioblastoma		File:IDH1_GBM_20x.jpg \| IDH-1 (R132H) in a secondary glioblastoma
			File:IDH1R132H oligoastrocytoma.jpg \| IDH-1 in a oliogoastrocytoma grade II
			File:Neuropathology case IX.jpg \| Absence of IDH-1 in a primary glioblastoma
	</gallery>		</gallery>

Jensflorian: IDH1 mutation specific antibody

2015-06-15T12:14:13Z

IDH1 mutation specific antibody

New page

[[File:IDH1_R132H_in_anaplastic_ologodendroglioma.jpg|thumb|right|250px|IDH1 staining in [[oligodendroglioma]].]]
'''Isocitrate dehydrogenase 1 (R132H)''', often abbreviated '''IDH1''' or '''IDH-1''' is a mutation-specific antibody detecting the R132H mutation in tumor material.<ref>{{Cite journal | last1 = Capper | first1 = D. | last2 = Zentgraf | first2 = H. | last3 = Balss | first3 = J. | last4 = Hartmann | first4 = C. | last5 = von Deimling | first5 = A. | title = Monoclonal antibody specific for IDH1 R132H mutation. | journal = Acta Neuropathol | volume = 118 | issue = 5 | pages = 599-601 | month = Nov | year = 2009 | doi = 10.1007/s00401-009-0595-z | PMID = 19798509 }}
</ref>

==Positive staining==
*Cytoplasmic<ref>{{Cite journal | last1 = Capper | first1 = D. | last2 = Weissert | first2 = S. | last3 = Balss | first3 = J. | last4 = Habel | first4 = A. | last5 = Meyer | first5 = J. | last6 = Jäger | first6 = D. | last7 = Ackermann | first7 = U. | last8 = Tessmer | first8 = C. | last9 = Korshunov | first9 = A. | title = Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. | journal = Brain Pathol | volume = 20 | issue = 1 | pages = 245-54 | month = Jan | year = 2010 | doi = 10.1111/j.1750-3639.2009.00352.x | PMID = 19903171 }}</ref>
<gallery>
File:IDH1_GBM_20x.jpg | IDH-1 (R132H) in a secondary glioblastoma
</gallery>

Note:
*The antibody does not detect other rare IDH-1/2 mutations (ie. R132G or R172K).

==Normal tissues==
*Absent

==Tumours==
*[[Oligodendroglioma]]<ref>{{Cite journal | last1 = Capper | first1 = D. | last2 = Reuss | first2 = D. | last3 = Schittenhelm | first3 = J. | last4 = Hartmann | first4 = C. | last5 = Bremer | first5 = J. | last6 = Sahm | first6 = F. | last7 = Harter | first7 = PN. | last8 = Jeibmann | first8 = A. | last9 = von Deimling | first9 = A. | title = Mutation-specific IDH1 antibody differentiates oligodendrogliomas and oligoastrocytomas from other brain tumors with oligodendroglioma-like morphology. | journal = Acta Neuropathol | volume = 121 | issue = 2 | pages = 241-52 | month = Feb | year = 2011 | doi = 10.1007/s00401-010-0770-2 | PMID = 21069360 }}</ref>
*[[Astrocytoma]]
*Secondary [[glioblastoma]]
*[[Myelodysplasia]]<ref>{{Cite journal | last1 = Andrulis | first1 = M. | last2 = Capper | first2 = D. | last3 = Luft | first3 = T. | last4 = Hartmann | first4 = C. | last5 = Zentgraf | first5 = H. | last6 = von Deimling | first6 = A. | title = Detection of isocitrate dehydrogenase 1 mutation R132H in myelodysplastic syndrome by mutation-specific antibody and direct sequencing. | journal = Leuk Res | volume = 34 | issue = 8 | pages = 1091-3 | month = Aug | year = 2010 | doi = 10.1016/j.leukres.2010.02.014 | PMID = 20227112 }}</ref>
*[[Acute myeloid leukemia]]

===Negative===
Negative staining:<ref>{{Cite journal | last1 = Capper | first1 = D. | last2 = Reuss | first2 = D. | last3 = Schittenhelm | first3 = J. | last4 = Hartmann | first4 = C. | last5 = Bremer | first5 = J. | last6 = Sahm | first6 = F. | last7 = Harter | first7 = PN. | last8 = Jeibmann | first8 = A. | last9 = von Deimling | first9 = A. | title = Mutation-specific IDH1 antibody differentiates oligodendrogliomas and oligoastrocytomas from other brain tumors with oligodendroglioma-like morphology. | journal = Acta Neuropathol | volume = 121 | issue = 2 | pages = 241-52 | month = Feb | year = 2011 | doi = 10.1007/s00401-010-0770-2 | PMID = 21069360 }}</ref>
*Non-tumorous tissue, endothelial cells.
*[[Ependymoma]]
*[[Pilocytic astrocytoma]]
*Neuronal tumors
*Sarcoma, carcinoma <ref>{{Cite journal | last1 = Sahm | first1 = F. | last2 = Capper | first2 = D. | last3 = Meyer | first3 = J. | last4 = Hartmann | first4 = C. | last5 = Herpel | first5 = E. | last6 = Andrulis | first6 = M. | last7 = Mechtersheimer | first7 = G. | last8 = Petersen | first8 = I. | last9 = Paulus | first9 = W. | title = Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation. | journal = Histopathology | volume = 58 | issue = 7 | pages = 1167-72 | month = Jun | year = 2011 | doi = 10.1111/j.1365-2559.2011.03823.x | PMID = 21707716 }}</ref>

==See also==
*[[Immunohistochemistry]].

==References==
{{Reflist|1}}

[[Category:Immunohistochemistry]]